Thermal Denaturation of Protein - UV Vis Spectroscopy

This spectroscopy application confirms the reversible process of protein denaturation with two distinguishable states. For more information, please proceed to download the application note below.

How is the thermal denaturation of protein determined spectrophotometrically?  

The thermal transition for a sample of chymotrypsinogen has been determined using UV Vis spectroscopy and the CuveT peltier thermostat by analyzing the absorbance over the temperature range between 20ºC to 85ºC. The reversible process of protein denaturation with two distinguishable states corresponding to the native and denatured with respect to temperature is confirmed.

For full materials, sample preparation, method, procedure and results analysis, please proceed to download the application note.